Serial dilutions are a common practice in the natural sciences.Due to the period decrease in concentration, this method is very useful when performing many types of experiments, from chemistry to biology to medicine.
Serial Dilution Microbiology Practice Serial Dilution IsSerial Dilution Microbiology Pdf Serial Dilution Microbiology Lab A serial dilution is the stepwise dilution of a substance in solution.Usually the dilution factor at each step is constant, resulting in a geometric progression of the concentration in a logarithmic fashion.
A ten-fold serial dilution could be 1 M, 0.1 M, 0.01 M, 0.001 M. A tenfold dilution for each step is called a logarithmic dilution or log-dilution, a 3.16-fold (10 0.5 -fold) dilution is called a half-logarithmic dilution or half-log dilution, and a 1.78-fold (10 0.25 -fold) dilution is called a quarter-logarithmic dilution or quarter-log dilution. Serial dilutions are widely used in experimental sciences, including biochemistry, pharmacology, microbiology, and physics. Oct 09, 2017 My video will introduce the steps of diluting a bacterial culture with the goal of determining how many live bacteria are present in that culture. Serial Dilution Microbiology Practice Series Of SequentialOct 08, 2013 A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. In biology and medicine edit In biology and medicine, besides the more conventional uses described above, serial dilution may also be used to reduce the concentration of microscopic organisms or cells in a sample. As, for instance, the number and size of bacterial colonies that grow on an agar plate in a given time is concentration-dependent, and since many other diagnostic techniques involve physically counting the number of micro-organisms or cells on specials printed with grids (for comparing concentrations of two organisms or cell types in the sample) or wells of a given volume (for absolute concentrations), dilution can be useful for getting more manageable results. Serial dilution is also a cheaper and simpler method for preparing cultures from a single cell than optical tweezers and micromanipulators. In homeopathy edit Serial dilution is one of the core foundational practices of homeopathy, with succussion, or shaking, occurring between each dilution. In homeopathy, serial dilutions (called potentisation) are often taken so far that by the time the last dilution is completed, no molecules of the original substance are likely to remain. See also edit References edit K. R. Aneja. Experiments in Microbiology, Plant Pathology and Biotechnology. New Age Publishers, 2005, p. ISBN81-224-1494-X Booth, C.; et al. Extremophiles. Methods in microbiology 35. The FASEB Journal. PMID16940145. Retrieved 2008-02-01. Trends in Pharmacological Sciences. CiteSeerX 10.1.1.385.5505. PMID16165225. Michael L. Bishop, Edward P. Fody, Larry E. Schoeff. Clinical Chemistry: Principles, Procedures, Correlations. Lippincott Williams Wilkins, 2004, p. ISBN0-7817-4611-6. External links edit Serial Dilution Microbiology Pdf How to Make Simple Solutions and Dilutions, Bates College Serial Dilution Microbiology Lab Retrieved from.
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